Indirect organogenesis of faba bean (Vicia faba L. Minor)

摘要

Using four Polish Vicia faba L. minor cultivars (Bronto, Dino, Tibo, Nadwi?lański) we obtained callus from epicotyl fragments collected from 7- and 14-day-old seedlings and from cotyledonary nodes of immature seeds. Callus induction efficiency varied from 81% to 97% depending on the origin of the explant. Shoots regenerated only from the cotyledonary nodes of all tested cultivars. On average, 50% of the explants grown on MS medium containing 1.0 mg dm~(-3) NAA, 0.5 mg dm~(-3) BAP, 0.25 mg dm~(-3) GA_3, 1.0 g dm~(-3) casein hydrolysate, 750 mg dm~(-3) inositol, 3% sucrose and 0.4% agar were able to regenerate shoots. The number of calluses regenerating shoots was highest from explants collected from fruiting nodes 6 to 9. Decapitation of donor plants increased the percentage of calluses regenerating shoots. On half-strength MS medium with 2 mg dm~(-3) NAA and on 1/2 MS alone, 11% of the shoots rooted; on 1/2 MS with 1 g dm~(-3) AC, 8.0% rooted. The regenerants were transferred to Perlite with Hoagland medium and acclimated. Ten percent of the regenerated plants survived the acclimation process, flowered and produced seeds.