DNA-loaded microbubbles with crosslinked bovine serum albumin shells for ultrasound-promoted gene delivery and transfection

摘要

Graphical abstractDisplay OmittedHighlights?BSA-based crosslinked positive microbubbles (CPMBs) were prepared.?CPMBs could electrostatically load DNA to form DNA-loaded microbubbles CPMBs/DNA.?CPMBs/DNA promoted gene expression more effectively than SonoVue under sonication.?CPMBs/DNA under sonication could promote and alter the way that gene entered cells.AbstractThe microbubble is a kind of clinically applied ultrasound contrast agent in disease diagnosis that can also rupture under sonication to increase membrane permeability and promote gene entry into targeted cells. However, the development of