Improved rate of callus and plantlet formation from anther culture of asparagus (Asparagus officinalis L.)

摘要

Asparagus anthers containing microspores at the early uninucleate to early-binucleate stages were successfully cultured on half-strength MS medium (without FeNa-EDTA) supplemented with 1.0 mg 2,4-D, 0.5-2.0 mg NAA and 0.5-2.0 mg benzyladenine/litrefor callus formation. The highest frequency of callus induction was 78.05%. Callus formation ability differed among individual plants. Organogenesis was induced when callus was subcultured on MS medium with 0.05-0.2 mg benzyladenine and 0.05-0.3 mg NAA/litre. The highest frequency of stem and pseudo-leaf induction (76.96%) was obtained on MS medium with 0.1 mg NAA and 0.1 mg benzyladenine/litre. A few calluses developed shoots and roots. Clustered shoots cultured on half-strength MS (without FeNa-EDTA)medium with 0.1 mg NAA or 2.0 mg IBA + 1.0-2.0 mg IAA or 2.0 mg IBA + 0.1 mg NAA or 1.5 mg IBA + 0.1 mg kinetin/litre initiated roots. The highest rooting percentage was 62.37% on medium with 0.1 mg NAA/litre. The regenerated plantlets were hardened under strong light for 15-20 days. They had normal pseudo-leaves and strong roots. The greatest transplanting survival rate was 84.55%.