CALLUS INDUCTION MEDIUM, SHOOT INDUCTION REGENERATION MEDIUM FOR CHRYSANTHEMUM ANTHER CULTURE, AND PREPARING METHOD OF CHRYSANTHEMUM HAPLOID PLANTLET BY ANTHER CULTURE USING THE SAME

摘要

The present invention relates to a medium for callus induction for culturing a Dendranthema grandiflorum anther, a differentiation medium for shoot induction, and a method for preparing a Dendranthema grandiflorum haploid plant by anther culture using the same. As a haploid plant can be converted into a diploid, to be efficiently used for a variety breeding material by combination of various pure lines, a medium for callus induction capable of culturing Dendranthema grandiflorum anther, a differentiation medium for shoot induction, and a method for preparing a Dendranthema grandiflorum haploid plant by anther culture using the same are provided. According to the present invention, before culturing Dendranthema grandiflorum anther, as Dendranthema grandiflorum anther is pre-treated at low temperatures and cultured in a medium having a specific composition, while a callus differentiation rate and a shoot induction rate of Dendranthema grandiflorum anther are improved, a Dendranthema grandiflorum haploid plant can be prepared.