Isolation of transposon Tn5 mutant affected in the metabolism of 18#beta#-glycyrrhetinic acid

摘要

Transposon Tn5 mutagenesis was carried out to clarify the metabolism of 18#beta-glycyrrhetinic acid (18#beta#-GRA) in Sphingomonas paucimobilis G5.A Tn5-induced mutant strain,named TM9638 that was affected in its metabolism of 18#beta#-GRA,was isolated.This mutant accumulated three metabolites,designated as M-A,M-B and M-C,from 18#beta#-GRA in the cultrure broth.M-A was accumulated in the culture broth of wild type strain.M-B and M-C were isolated from the culture broth of TM9638 and the chemical structures were elucidated by NMR and GC-MS.