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Expression of ESAT‐6 antigen from Mycobacterium tuberculosisMycobacterium tuberculosis in broccoli: An edible plant

机译：Esat-6抗原的表达 结核分枝杆菌 - 结核分枝杆菌 在西兰花：可食用的植物

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Abstract > Tuberculosis (TB) is one of the major infectious diseases caused by Mycobacterium tuberculosis . The development of an effective and economical vaccine for controlling TB is essential especially for developing countries. Edible plants can serve as biofactories to produce vaccine antigens. In this study, 6?kDa early secretory antigenic target (ESAT‐6) of M. tuberculosis was expressed in Brassica oleracea var. italica via Agrobacterium ‐mediated transformation to facilitate oral delivery of antigen. ESAT‐6 gene was cloned using Gateway? cloning strategy. Transformation and presence of transgene was confirmed through PCR. Expression level of transgene was calculated via quantitative real‐time PCR (qRT‐PCR) and the maximum integrated transgene number was two. Maximum amount of total soluble fraction of ESAT‐6 was evaluated by immunoblotting, estimated to accumulate up to 0.5% of total soluble protein. The recombinant ESAT‐6 protein was further purified and detected using silver staining and Western blotting. ESAT‐6 protein induced humoral immune response in mice immunized orally and subcutaneously. The expression of M. tuberculosis antigen in edible plants could aid in the development of cost‐effective and oral delivery of an antigen‐based subunit vaccine against TB. To the best our knowledge, it is the first report of expression of a vaccine antigen in broccoli. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 结核病（TB）是由此引起的主要传染病之一 分枝杆菌结核病</ i> 。用于控制TB的有效和经济疫苗的开发至关重要，特别适用于发展中国家。可食用的植物可以用作生产疫苗抗原的生物暗症。在本研究中，6？KDA早期分泌抗原靶（ESAT-6） m。结核病</ i> 表达了 芸苔糖酸乙片</ i> var。 italica </ i> 通过 农杆菌</ i> 介导的转化，以促进口服递送抗原。使用网关克隆ESAT-6基因？克隆策略。通过PCR确认转基因的转化和存在。通过定量实时PCR（QRT-PCR）计算转基因的表达水平，并且最大集成的转基因数为2。通过免疫印迹评估ESAT-6的总可溶性级分的最大量，估计积聚可加速可溶性蛋白质的0.5％。进一步纯化重组ESAT-6蛋白，并使用银染色和蛋白质印迹检测。 ESAT-6蛋白在口服和皮下免疫的小鼠中诱导体液免疫应答。表达 m。结核病</ i> 可食用植物中的抗原可以有助于开发抗原的抗原的亚基疫苗的成本效益和口服递送。据我们所知，它是西兰花疫苗抗原表达的第一报告。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2020年第1期</span><b style="margin: 0 2px;">|</b><span>共10页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Agrobacterium ‐mediated transformation&option=203" rel="nofollow">Agrobacterium ‐mediated transformation;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=edible vaccines&option=203" rel="nofollow">edible vaccines;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=ESAT‐6&option=203" rel="nofollow">ESAT‐6;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gateway? cloning&option=203" rel="nofollow">Gateway? cloning;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=humoral immune response&option=203" rel="nofollow">humoral immune response;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Mycobacterium tuberculosis&option=203" rel="nofollow">Mycobacterium tuberculosis;</a> </p> <div class="translation"> 机译：农杆菌介导的转化;可食用疫苗;ESAT-6;网关？ 克隆;体液免疫应答;结核分枝杆菌; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881050.html">Expression of ESAT‐6 antigen from <i >Mycobacterium tuberculosisMycobacterium tuberculosis in broccoli: An edible plant</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2020</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：Esat-6抗原的表达 结核分枝杆菌 - 结核分枝杆菌 在西兰花：可食用的植物</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_chinese-medical-journal_thesis/0201254797026.html">Expression and immunogenicity of recombinant Mycobacterium bovis Bacillus Calmette-Guérin strains secreting the antigen ESAT-6 from Mycobacterium tuberculosis in mice</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=WANG Li-mei&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">WANG Li-mei,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=SHI Chang-hong&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">SHI Chang-hong,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=FAN Xiong-lin&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">FAN Xiong-lin,</a> <a href="/journal-foreign-6708/" target="_blank" rel="nofollow" class="tuijian_authcolor">中华医学杂志（英文版） .</a> <span>2007</span><span>,第014期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：小鼠结核分枝杆菌分泌抗原ESAT-6的重组牛分枝杆菌卡介苗的表达和免疫原性</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204121378098.html">Early Secreted Antigenic Target of 6 kDa (ESAT-6) Protein of Mycobacterium tuberculosis Induces Interleukin-8 (IL-8) Expression in Lung Epithelial Cells via Protein Kinase Signaling and Reactive Oxygen Species</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Vijay Boggaram&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Vijay Boggaram,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Koteswara R. 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href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨秀旭&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨秀旭</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李浩&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李浩</a> <span> <a href="/journal-cn-5085/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生物技术通讯 </a> </span> <span> . 2011</span><span>,第002期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-21824_thesis/02022466732.html">结核分枝杆菌分泌性蛋白ESAT-6和CFP-10在植物系统中的表达</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=曾献武&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 曾献武</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张珂&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张珂</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=李春阳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,李春阳</a> <span> <a href="/conference-cn-21824/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第九次全国生物制品学术会议 </a> <span> <span> . 2007</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020311228955.html">鹿源牛结核分枝杆菌ESAT-6、Ag85b蛋白的表达与纯化及抗原特异性研究</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=谷晨晨&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 谷晨晨</a> <span> . 2018</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120924982.html">可表达结核分枝杆菌Ag85B和ESAT-6融合蛋白的重组耻垢分枝杆菌株及其应用</a> <b>[P]</b> . <span> 中国专利： CN101875913B </span> <span> . 2012.05.23</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/06120106056199.html">可表达结核分枝杆菌Ag85B和ESAT-6融合蛋白的重组耻垢分枝杆菌株及其应用</a> <b>[P]</b> . <span> 中国专利： CN101875913A </span> <span> . 2010-11-03</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130419229256.html">RECOMBINANT PLASMID pBi121-ESAT6-CFP10-gIFN, CODING PROTEINS ESAT6 AND CFP10 MYCOBACTERIUM TUBERCULOSIS AND HUMAN GAMMA-INTERFERON IN TRANSGENIC PLANTS</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2468082C1 </span> <span> . 2012-11-27</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：转基因植物中重组质粒pBi121-ESAT6-CFP10-gIFN，编码蛋白ESAT6和CFP10结核分枝杆菌和人类γ-干扰素 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130403051953.html">RECOMBINANT INFLUENZA VIRUS STRAIN A/PR8/HK-NS80E85A, EXPRESSING FRAGMENTS OF ESAT-6 AND AG85A MYCOBACTERIUM TUBERCULOSIS ANTIGENS, TO OBTAIN TUBERCULOSIS VECTORED VACCINE</a> <b>[P]</b> . <span> 外国专利： <!-- 俄罗斯专利： --> RU2678175C1 </span> <span> . 2019-01-23</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：重组流感病毒株A / PR8 / HK-NS80E85A，表达ESAT-6和AG85A结核分枝杆菌抗原片段，获得结核分枝杆菌疫苗 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130414542948.html">PLASTID-EXPRESSED MYCOBACTERIUM TUBERCULOSIS VACCINE ANTIGENS ESAT-6 AND MTB72F FUSED TO CHOLERA TOXIN B SUBUNIT</a> <b>[P]</b> . <span> 外国专利： <!-- 欧洲知识产权局专利： --> EP2771469A4 </span> <span> . 2015-04-08</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：质体表达结核分枝杆菌结核分枝杆菌疫苗抗原ESAT-6和MTB72F融合至霍乱毒素B亚单位 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: 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