• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biotechnology and Applied Biochemistry >Reconstruction and analysis of a three‐compartment genome‐scale metabolic model for Pseudomonas fluorescensPseudomonas fluorescens
【24h】

Reconstruction and analysis of a three‐compartment genome‐scale metabolic model for Pseudomonas fluorescensPseudomonas fluorescens

机译：三室基因组代谢模型的重建与分析 假单胞菌荧光荧光荧光荧光剂

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract > With the versatile metabolic diversity, Pseudomonas fluorescens is a potential candidate in petroleum aromatic hydrocarbon (PAH) bioremediation. Genome‐scale metabolic model (GSMM) can provide systematic information to guide the development of metabolic engineering strategy to improve microbial activity. In this study, a GSMM for P.?fluorescens SBW25 was reconstructed, which is termed as lCW1057. The reconstruction was based on automatic reannotation and manual curation. The periplasmic compartment was constructed to better represent the proton gradient profile. The reconstructed proton transport chain has a P/O (ATP generated per atom oxygen consumed by the respiratory chain) ratio of 11/8. Flux balance analysis (FBA) was performed to explore the whole‐cell metabolic flow. The model suggested that instead of Embden–Meyerhof–Parnas pathway, Entner–Doudoroff pathway was used in glycolytic metabolism of P.?fluorescens , indicating that the growth of P.?fluorescens is more energy dependent. Furthermore, P.?fluorescens can use nitrate as the terminal electron acceptor for the glucose metabolism. The β‐ketoadipate pathway was involved in catechol metabolism. The uptake of oxygen is mandatory for the aromatic ring cleavage. The in silico and in vitro maximum specific growth rate was compared, resulting in 10% difference when catechol was used as the sole carbon source. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> > 随着多功能的代谢多样性， 假单胞菌荧光荧光素</ i> 是石油芳烃（PAH）生物化的潜在候选物。基因组级代谢模型（GSMM）可以提供系统信息，以指导代谢工程策略的发展，以改善微生物活动。在这项研究中，一个GSMM p.?fluorescens </ i> 重建SBW25，被称为LCW1057。重建是基于自动的成果和手动策策。构建过周质室以更好地代表质子梯度曲线。重建的质子传输链具有11/8的P / O（呼吸链消耗的每次原子氧产生的ATP）的比例。进行通量平衡分析（FBA）以探讨全细胞代谢流。该模型建议，而不是Embden-Meyerhof-Parnas途径，Entner-doudoroff途径用于糖酵解代谢 p.?fluorescens </ i> ，表明增长了 p.?fluorescens </ i> 更有能量依赖。此外， p.?fluorescens </ i> 可以使用硝酸盐作为葡萄糖代谢的末端电子受体。 β-酮饼型途径参与儿茶酚代谢。对于芳香环切割是强制性的氧气的吸收。这 在Silico </ i> 和 在体外</ i> 比较最大的比较速率，导致儿茶酚用作唯一碳源时10％的差异。 </ p> </摘要> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-15016/'>《Biotechnology and Applied Biochemistry》</a> <b style="margin: 0 2px;">|</b><span>2020年第1期</span><b style="margin: 0 2px;">|</b><span>共7页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/180.html" title="生物工程学（生物技术）">生物工程学（生物技术）;</a><a href="https://www.zhangqiaokeyan.com/clc/177.html" title="生物化学">生物化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=genome‐scale metabolic model&option=203" rel="nofollow">genome‐scale metabolic model;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Pseudomonas fluorescens&option=203" rel="nofollow">Pseudomonas fluorescens;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=catechol&option=203" rel="nofollow">catechol;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=bioremediation&option=203" rel="nofollow">bioremediation;</a> </p> <div class="translation"> 机译：基因组级代谢模型;荧光荧光;儿茶酚;生物修复; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704021881016.html">Reconstruction and analysis of a three‐compartment genome‐scale metabolic model for <i >Pseudomonas fluorescensPseudomonas fluorescens</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> </a> <a href="/journal-foreign-15016/" target="_blank" rel="nofollow" class="tuijian_authcolor">Biotechnology and Applied Biochemistry .</a> <span>2020</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：三室基因组代谢模型的重建与分析 假单胞菌荧光荧光荧光荧光剂</span> </p> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204113968119.html">Mapping global effects of the anti-sigma factor MucA in Pseudomonas fluorescens SBW25 through genome-scale metabolic modeling</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sven EF Borgos&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sven EF Borgos,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sergio Bordel&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sergio Bordel,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=H?vard Sletta&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">H?vard Sletta,</a> <a href="/journal-foreign-15098/" target="_blank" rel="nofollow" class="tuijian_authcolor">BMC Systems Biology .</a> <span>2013</span><span>,第1期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过基因组规模的代谢模型绘制荧光假单胞菌SBW25中抗sigma因子MucA的整体效应</span> </p> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-foreign_detail_thesis/0204116951572.html">redGEM: Systematic reduction and analysis of genome-scale metabolic reconstructions for development of consistent core metabolic models</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Meric Ataman&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Meric Ataman,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Daniel F. Hernandez Gardiol&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Daniel F. Hernandez Gardiol,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Georgios Fengos&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Georgios Fengos,</a> <a href="/journal-foreign-29516/" target="_blank" rel="nofollow" class="tuijian_authcolor">PLoS Computational Biology .</a> <span>2017</span><span>,第7期</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：redGEM：系统化减少和分析基因组规模的代谢重建，以开发一致的核心代谢模型</span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-conference-foreign_meeting-258995_thesis/0705012936779.html">Merlin: Metabolic Models Reconstruction using Genome-Scale Information</a> <b>[C]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Oscar Dias&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Oscar Dias,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Miguel Rocha&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Miguel Rocha,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eugenio C. Ferreira&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Eugenio C. Ferreira,</a> <a href="/conference-foreign-258995/" target="_blank" rel="nofollow" class="tuijian_authcolor">IFAC Symposium on Computer Applications in Biotechnology .</a> <span>2010</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：Merlin：使用基因组信息重建代谢模型</span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-degree-foreign_mphd_thesis/02061377638.html">A Framework for the Reconstruction and Analysis of Tissue Specific Genome-Scale Metabolic Models</a> <b>[D] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Correia, Sara Alexandra Gomes.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Correia, Sara Alexandra Gomes. </a> <span>2016</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：组织特异性基因组规模代谢模型的重建和分析框架</span> </p> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-journal-foreign-pmc_detail_thesis/040003229215.html">Mapping global effects of the anti-sigma factor MucA in Pseudomonas fluorescens SBW25 through genome-scale metabolic modeling</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sven EF Borgos&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sven EF Borgos,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sergio Bordel&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sergio Bordel,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Håvard Sletta&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Håvard Sletta,</a> <span>2013</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过基因组规模的代谢模型绘制荧光假单胞菌SBW25中抗Sigma因子MucA的整体效应</span> </p> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/open-access_resources_thesis/0100010365764.html">Mapping global effects of the anti-sigma factor MucA in Pseudomonas fluorescens SBW25 through genome-scale metabolic modeling</a> <b>[O] </b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Borgos Sven Even F.&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Borgos Sven Even F.,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Bordel Sergio&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Bordel Sergio,</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Sletta Håvard&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">Sletta Håvard,</a> <span>2015</span> </span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：通过基因组规模的代谢模型绘制荧光假单胞菌SBW25中抗Sigma因子MucA的整体效应</span> </p> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/academic-journal-cn_china-surfactant-detergent-cosmetics_thesis/0201286550565.html">洗涤剂用荧光增白剂 第三讲 洗涤剂中荧光增白剂的分析</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵章楣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 赵章楣</a> <span> <a href="/journal-cn-9182/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 日用化学工业 </a> </span> <span> . 1981</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-jiangsu-police-institute_thesis/020122502704.html">荧光产生及指纹荧光显现剂目标分子模型设计</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘景宁&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘景宁</a> <span> <a href="/journal-cn-196/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 江苏警官学院学报 </a> </span> <span> . 2005</span><span>,第003期</span> </span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/academic-journal-cn_guangdong-chemical-industry_thesis/0201246297884.html">反向毛细管电泳-激光诱导荧光快速分析面粉中的荧光增白剂</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=罗军漫&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 罗军漫</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=韦萍&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,韦萍</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=袁凯松&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,袁凯松</a> <span> <a href="/journal-cn-9122/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 广东化工 </a> </span> <span> . 2017</span><span>,第005期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_drilling-fluid-completion_thesis/0201219240418.html">用OFA-2石油荧光分析仪测定钻井液添加剂荧光的方法</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=赵菊英&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 赵菊英</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=王欣&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,王欣</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=苏慧敏&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,苏慧敏</a> <span> <a href="/journal-cn-7938/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 钻井液与完井液 </a> </span> <span> . 2003</span><span>,第006期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-journal-cn_journal-hainan-university-natural-science-edition_thesis/0201267326320.html">应用LS—50B荧光分光光度计进行洗衣粉增白剂的荧光分析法</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=詹道松&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 詹道松</a> <span> <a href="/journal-cn-51201/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 海南大学学报：自然科学版 </a> </span> <span> . 1995</span><span>,第001期</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-21781_thesis/02022454681.html">荧光定量PCR在铜绿假单胞菌败血症模型中运用的研究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨曼琼&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 杨曼琼</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=钟礼立&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,钟礼立</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=张兵&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,张兵</a> <span> <a href="/conference-cn-21781/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 第七次全国临床微生物学术年会暨第三届两岸三地临床微生物与感染病学术论坛 </a> <span> <span> . 2007</span> </span> </div> </li> <li> <div> <b>7. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020314527100.html">分子荧光光谱法在荧光增白剂分析中的应用</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=唐容&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 唐容</a> <span> . 2016</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06120104618888.html">利用荧光嵌入剂对双链和三链核酸在溶液中杂交的荧光强度分析</a> <b>[P]</b> . <span> 中国专利： CN1413264A </span> <span> . 2003-04-23</span> </div> </li> <li> <div> <b>2. </b><a class="enjiyixqcontent" href="/patent-detail/061203229084.html">一种同时分析人基因组DNA 24个基因座的荧光标记复合扩增的试剂盒及其应用</a> <b>[P]</b> . <span> 中国专利： CN105385763B </span> <span> . 2019.03.05</span> </div> </li> <li> <div> <b>3. </b><a class="enjiyixqcontent" href="/patent-detail/06130427547019.html">GENOME-SCALE BUTANOL-PRODUCING MICROORGANISM METABOLIC NETWORK MODEL AND METHODS USING THE SAME FOR ANALYSIS OF METABOLIC CHARACTERISTICS OF BUTANOL-PRODUCING MICROORGANISMS AND FOR SCREENING DELETION TARGETS</a> <b>[P]</b> . <span> 外国专利： <!-- 世界知识产权组织专利： --> WO2009128644A3 </span> <span> . 2010-01-21</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基因组规模的生产丁醇的微生物代谢网络模型和方法，用于分析生产丁醇的微生物的微生物代谢特征和筛选缺失目标 </span> </p> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/patent-detail/06130427547018.html">GENOME-SCALE BUTANOL-PRODUCING MICROORGANISM METABOLIC NETWORK MODEL AND METHODS USING THE SAME FOR ANALYSIS OF METABOLIC CHARACTERISTICS OF BUTANOL-PRODUCING MICROORGANISMS AND FOR SCREENING DELETION TARGETS</a> <b>[P]</b> . <span> 外国专利： <!-- 世界知识产权组织专利： --> WO2009128644A9 </span> <span> . 2010-04-15</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基因组规模的生产丁醇的微生物代谢网络模型和方法，用于分析生产丁醇的微生物的微生物代谢特征和筛选缺失目标 </span> </p> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/patent-detail/06130429677532.html">GENOME-SCALE BUTANOL-PRODUCING MICROORGANISM METABOLIC NETWORK MODEL AND METHODS USING THE SAME FOR ANALYSIS OF METABOLIC CHARACTERISTICS OF BUTANOL-PRODUCING MICROORGANISMS AND FOR SCREENING DELETION TARGETS</a> <b>[P]</b> . <span> 外国专利： <!-- 世界知识产权组织专利： --> WO2009128644A2 </span> <span> . 2009-10-22</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：基因组规模的生产丁醇的微生物代谢网络模型和方法，用于分析生产丁醇的微生物的微生物代谢特征和筛选缺失目标 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704021881016','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> <h2>期刊订阅</h2> <img src="https://cdn.zhangqiaokeyan.com/img/loginclose.png" alt="" onclick="$('.journalsub-pop-up').hide()"> <p class="pardon">抱歉，该期刊暂不可订阅，敬请期待！</p> <p class="current">目前支持订阅全部北京大学中文核心（2020）期刊目录。</p> <div style="display: flex;margin-top: 69px;justify-content: space-between;"> <div class="no-sub" onclick="$('.journalsub-pop-up').hide()">暂不订阅</div> <div class="other-sub" onclick="continueSub('from=pc-detail')">继续订阅其他期刊</div> </div> </div> </div> <div class="right_btn"> <ul> <li class="gouwuche"> <!-- <a href="javascript:void(0);" onclick="link_analysis('/shoppingcart/auth/list.html',this)">购物车</a>--> </li> <li class="yijian"> <a href="javascript:void(0);" onclick="link_analysis('/mycenter/auth/complaint.html',this)">意见反馈</a> </li> <li class="top"> <a href="javascript:scrollTo(0,0);">回到顶部</a> </li> <li class="shouye"> <a href="/">回到首页</a> </li> </ul> </div> <div class="xllindexfooter"> <div class="xllindexfootercenter"> <div class="xllindexfooterleft left" > <div class="xllindexfooterleftli"> <ul> <li><a href="/about.html">关于掌桥</a></li> <li><a href="/help/helpmap.html">资源导航</a></li> <li><a href="/help/helpguide.html">新手指南</a></li> <li><a href="/help/helpcenter.html">常见问题</a></li> <li><a href="/sitemap.html">网站地图</a></li> <li><a href="/help/helpcenter.html?type=9">版权声明</a></li> </ul> </div> <div class="xllindexfooterleft"> <p class="xllindexfooterlefteamil">客服邮箱：kefu@zhangqiaokeyan.com</p> <div class="xllindexfooterlefttcp"> <div class="xllindexfooterpoliceiimg"></div> <div class="xllindexfooterpoliceispan"> <span>京公网安备：11010802029741号 </span> <span>ICP备案号：<a href="https://beian.miit.gov.cn" rel="nofollow" target="_blank">京ICP备15016152号-6</a></span> <span>六维联合信息科技 (北京) 有限公司©版权所有</span> </div> </div> </div> </div> <div class="xllindexfooterright left"> <ul> <li> <p style="font-weight: bold;">客服微信</p> <div></div> </li> <li> <p style="font-weight: bold;">服务号</p> <div></div> </li> </ul> </div> </div> </div> <span id="0704021881016down" data-source="7," data-out-id="XBoeztzMu/BpX0EIuslVWvdPwhn/xzTe1vNOtRNkZH3rE1IazGzh4LbmysdgdMwC," data-f-source-id="7" data-title="Reconstruction and analysis of a three‐compartment genome‐scale metabolic model for <i >Pseudomonas fluorescensPseudomonas fluorescens" data-price="20" data-site-name="" data-transnum="24" style="display:none;"></span> <input type="hidden" value="4" id="sourcetype"> <input type="hidden" value="15016" id="journalid"> <input type="hidden" value="1" id="inyn_provide_service_level"> <input type="hidden" value="https://cdn.zhangqiaokeyan.com" id="imgcdn"> <input type="hidden" value="1" id="isdeatail"> <input type="hidden" value="" id="syyn_indexed_database"> <input type="hidden" value="" id="servicetype"> <input type="hidden" id="pagename" value="Reconstruction and analysis of a three‐compartment genome‐scale metabolic model for <i >Pseudomonas fluorescensPseudomonas fluorescens"/> <input type="hidden" value="thesis_get_original" id="pageIdentification"> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery-1.12.4.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/lwlh_ajax.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zq.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/common.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/jquery.cookie.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/top.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/tip.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/login.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/down.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/search.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/searchtype.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/user/regist.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/zxs_solor/detail.js?v=5.7.7"></script> <script type="text/javascript" src="https://www.zhangqiaokeyan.com/statistics/static/pagecollection.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/tj.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/pushbaidu.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/common/history.js"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/util/cookie.js?v=5.7.7"></script> <script type="text/javascript" src="https://cdn.zhangqiaokeyan.com/js/weipu/weipu.js?v=5.7.7"></script> </body> <script> $(function(){ var weiPuStatus = getCookie('WeiPuStatus'); if(weiPuStatus){ tipWeiPuStatus(weiPuStatus); delCookie('WeiPuStatus'); } getFacetKeywordVoInId(); var sourcetype = $("#sourcetype").val(); var inyn_provide_service_level = $("#inyn_provide_service_level").val(); if((sourcetype ==1||sourcetype==4)&&inyn_provide_service_level!=4){ getJournal(); } }) </script> </html>