Isolation of soil bacteria for bioremediation of hydrocarbon contamination

摘要

The study was conducted to identify the capability of the bacterial strains to degrade the crude oil in in vitro conditions. The soil samples were collected from oil contaminated sites at Aravakurichy Karur district, Tamilnadu. Soil samples were usedto analyze the moisture content, temperature, pH, total nitrogen, phosphorous, BOD and COD. From the soil sample, three dominant bacterial strains were isolated viz, Bacilius subtiiis, Pseudomonas aeruginosa, and Klebsiella pneumoniae. The isolates werescreened for its ability to degrade crude oil and all the Isolates showed maximum degradation. The degrading ability was higher in liquid medium when compared to solid media. Among the three isolated bacterial strains, P. aeruginosa showed higher degrading ability than the other twra isolates. A detailed analysis of the hydrocarbon extract was performed by Gas Chromatography