Oligophilic bacterial diversity of Leh soils and its characterization employing ARDRA

摘要

Oligophiles from uncultivated, mustard-, potato- and wheat-cultivated soils of Leh were studied. The substrate concentration of the standard plate count (SPC) medium was 1.75-0.0035% to facilitate the growth of copiotrophs and oligophiles. The ratio of bacterial counts on 1/20th dilution to full strength SPC was 3.26 in uncultivated and 1.74, 1.83 and 2.7, in wheat-, mustard- and potato-cultivated soil, respectively. ARDRA pattern with HaeIII of 36 randomly picked isolates showed a dissimilarity coefficient of 3.0. Despite the nature of the soil, seven phylogenetic groups were formed, which consisted of isolates from higher dilutions (1/20 and 1/100). Only three isolates recovered on normal SPC showed similarity with these isolates. This confirms that bacteria growing at higher dilutions are different. It is also clear that oligophiles are abundant in nature and the need is to develop methodologies to explore their existence.