Quantification of furanic derivatives in fortified wines by a highly sensitive and ultrafast analytical strategy based on digitally controlled microextraction by packed sorbent combined with ultrahigh pressure liquid chromatography

摘要

An improved, reliable and powerful analytical strategy based on digitally controlled microextraction by packed sorbent (MEPS) combined with ultrahigh pressure liquid chromatography ((JHPLC) was validated for the simultaneous identification and quantification of major furanic derivatives, namely 5-hydroxymethyl-2-furaldehyde (5HMF), 5-methyl-2-furaldehyde (5MF), 2-furaldehyde (2F) and 2-furyl methyl ketone (2FMK), in fortified wines. To enhance the extraction efficiency of the target furanic derivates, several influencing extraction parameters, such as number of loading cycles, nature of elution solvent and elution volume, were evaluated and optimized. In addition the ability of different MEPS sorbent materials, namely C2, C8, C18, SIL, M1, R-AX, R-OC and PGC, were also tested. The optimal analytical conditions involved loading 3 x 200 mu L of wine samples through a C8 sorbent in a MEPS syringe placed in the semi-automatic eVolH syringe followed by elution using 200 mu L MeOH:H2O (95:5, v/v). The furanic derivates separation was achieved using a CORTECS UPLC (R) C18 analytical column in an ultrafast chromatographic run (within 4min). The method performance was assessed for dry/medium dry (D/MD) and sweet/medium sweet (S/MS) model wines in terms of selectivity, linearity, limit of detection (LOD), limit of quantitation (LOQ), accuracy, precision and matrix effect, using model wine matrix-matched calibration. Good linearity was obtained with a regression coefficient (r(2)) higher than 0.992. A good precision was attained (RSD < 5%) and low LODs were achieved for D/MD (4.5-129.3 ng L-1) and S/MS (6.9-285.2 ng L-1) model wines. The quantification limits (LOQ) for D/MD model wines ranged from 14.9 to 431.0 ng L-1, whereas for S/MS model wines range from 23.1 to 950.5 ng L-1. The method also afforded satisfactory results in terms of accuracy, ranging from 74 to 97% for D/MD wines and between 84 and 99% for S/MS wines.