Determination of 12 Type A and B Trichothecenes in Cereals by Liquid Chromatography-Electrospray lonization Tandem Mass Spectrometry

摘要

A new sensitive method for the simultaneous determination of 12 trichothecenes(deoxynivalenol,nivalenol,3-acetyldeoxynivalenol,15-acetyldeoxynivalenol,fusarenon X,T-2 toxin,HT-2 toxin,neosolaniol,monoacetoxyscirpenol,diacetoxyscirpenol,T-2 triol,and T-2 tetraol)by liquid chroma-tography-electrospray ionization tandem mass spectrometry(LC-ESI-MS/MS)is presented.The development of the method and investigations on the matrix influence on the MS signal are described in particular.The matrix effect was thereby minimized by using an internal standard,a special mobile phase,and specific fragmentation parameters.The sample was extracted with acetonitrile/water(84:16,v/v),and the extract was cleaned up with a MycoSep 227 column.Quantification was based on the internal standard de-epoxy-deoxynivalenol.Calibration curves were linear between 16 and 1600 ng/g,and the limits of detection ranged from 0.18 to 5.0 ng/g.The developed method was applied for the determination of trichothecenes in 120 naturally contaminated wheat and oat samples.