Retention of nativelike conformation by proteins embedded in high external electric fields

摘要

In this Communication,we show that proteins embedded in high external electric fields are capable of retaining a nativelike fold pattern.We have tested the metalloprotein azurin,immobilized onto SiO_2 substrates in air with proper electrode configuration,by applying static fields up to 10~6-10~7 V/m.The effects on the conformational properties of protein molecules have been determined by means of intrinsic fluorescence measurements.Experimental results indicate that no significant field-induced conformational alteration occurs.Such results are also discussed and supported by theoretical predictions of the inner protein fields.