Antibody competition test (act) for the qualitative and conformation dependent quantitative detection of the conformation changes of p-glycoprotein and other cell surface receptors, pumps and proteins, and uses thereof

摘要

The invention relates to the detection of the presence and/or conformation change of the cell surface receptors, pumps or proteins, when two different antibodies and optionally ligands are bound to the receptors, pumps and/or proteins recognized by the antibodies, and the change in the antibody-binding is measured. The method can be used for the determination of cytostatic drug combination, effective in case of multidrug resistance wherein the native cells are combined in vitro with the drug or drug combination to be tested, and the sample is tested with the method mentioned above. The method can be used for the detection of the efficiency of multidrug-resistance modulators wherein the method according is carried out in the presence of known modulator or a modulator to be tested, and the resulting activity values are compared. Also the object of the invention is a diagnostic kit for the detection of the presence and conformation change of receptors, pumps and proteins, for the determination of the efficient drug combination in case of multidrug resistance and/or for the detection of the efficiency of the modulators comprising a first antibody which is able to detect the receptors, pumps or proteins, optionally other ligands, and a system, which provides a system for the detection of binding.