Pharmacokinetics of Colon-Targeted Pellets of Pulsatilla chinensis (Bunge) Regel Saponins-Hydroxypropyl-β-cyclodextrin Inclusion in Ratsf

摘要

The colon-targeted pellets of Pulsatilla chinensis saponins-hydroxypropyl-β-cyclodextrin inclusion was investigated for the pharmacokinetics study in rats. A rapid and sensitive assay was developed and validated using liquid chromatography-mass spectrometry (LC-MS) for Pulsatilla saponin D in rat plasma. Pulsatilla saponin D was extracted with acetonitrile from rat plasma samples and the supernatants was dried in a gentle stream of nitrogen and redissolved with methanol. It was separated using HPLC with a reversed phase column and analyzed by selected ion monitoring (SIM) at m/z of 935.4 and 969.4 for Ginsenoside Re as an internal standard. The Pulsatilla saponin D concentration was measured in plasma samples up to 24 h following oral administration of Pulsatilla chinensis saponins and the colon-targeted pellets of Pulsatilla chinensis saponins-hydroxypropyl-β-cyclodextrin inclusion both at a dose of 200 mg kg~(-1). The findings indicate that it can decrease the absorption rate and maximum concentration, at the same time improve the bioavailability by preparing colon-targeted pellets of hydroxypropyl-β-cyclodextrin inclusion complex.