Comparative study of desferrioxamine and deferiprone protects against aluminum induced compositional, structural and functional changes in liver tissue of mice (mus musculus) investigated by FT-IR spectroscopy

摘要

The present study was designed to investigate the protective effects of the chelating agents desferrioxamine (DFO) and deferiprone (DFP) in aluminum intoxicated liver tissue of mice by Fourier transform infrared (FT-IR) spectroscopy. The finding reveals the alterations on the major biochemical constituents, such as lipids, proteins, collagen, glycogen and nucleic acids of liver tissue at molecular level. The significant decreased in the peak areas of CH3 asymmetric and CH2 symmetric groups from control 0.120±0.073 and 0.924±0.041 to aluminum intoxicated 0.023±0.003 and 0.111±0.006, but treated with chelating agents DFP and DFO+DFP improved from 0.055±0.006 and 0.345±0.077 to 0.091±0.005 and 0.671±0.046 respectively for near to control values. This result suggests that due to aluminum poisoning decreased the lipids contents in the biological system. The bands ratio at I2958/I2850 significantly decreased from control (0.380±0.003) to aluminum (0.292±0.013), but improved it by DFP (0.323±0.002) and DFO+DFP (0.370±0.001) respectively. This decreased ratio indicates a decrease in the number of methyl groups in protein fibers compared to methylene groups in aluminum intoxicated liver tissue. The significant decreased in the peak areas of amide I and amide II groups from control 3.362±0.152 and 1.980±0.225 to aluminum intoxicated 0.713±0.022 and 0.258±0.020, but treated with DFP and DFO+DFP enhanced from 1.428±0.140 and 0.763±0.024 to 2.281±0.144 and 1.283±0.046 respectively for near to control values. This result suggests an alteration in the protein profile. Further, the absence of olefinicCH stretching band in aluminium exposure liver suggests an altered lipid levels. Therefore, FTIR can be used successfully applied to toxicological studies at molecular level.