Determination of amprolium, ethopabate, lasalocid, monensin, narasin, and salinomycin in feed by liquid chromatography-mass spectrometry

摘要

A liquid chromatographic-atmospheric pressure ionization ion spray method for the determination of six coccidiostatics in feed is presented. Feed samples were homogenized with methanolacetone-tetrahydrofuran. After addition of water, the samples were mixed and centrifuged. The compact bottom layer was re-extracted with methanol-water. After centrifugation, the combined supernatants were diluted and filtered through a Spin-X micro-centrifuge tube. Three different analytical columns were used. The calibration curves were linear in the investigated areas. The described assay offers a number of significant advantages compared to previously published methods for the detection and quantification of some coccidiostatics in feed. No derivatization is required.