METHOD FOR THE DETERMINATION OF ANTI HIV DRUGS IN PLASMA WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY

摘要

The invention chemical properties different plurality of AIDS treatment by high-performance liquid chromatography-mass spectrometry (High Performance Liquid Chromatography-Mass Spectrometry; LC-MS) for use by both the analysis to be in a high-performance liquid chromatography-mass spectrometry for using plasma relates to an AIDS therapeutic agent in the detection method, more specifically as an AIDS therapeutic agent detection method of the plasma, the liquid using a non-polar organic solvent in a sample of blood plasma-separating performing a liquid extraction and then centrifuged to obtaining a the supernatant non-polar organic solvent layer first step; A second step of removing precipitated the plasma proteins in the separated water layer in the first step to obtain a supernatant; And the sample plus the obtained supernatant in the supernatant and the second phase obtained in the first step of the high-performance liquid chromatography-high performance liquid chromatography, characterized in that it comprises a third step of quantitative analysis in the mass spectrometer - mass spectrometry It relates to a method for detecting AIDS treatment in plasma using. ; The detection method of the present invention chemical properties HIV (AIDS) therapeutic agent for high performance liquid chromatography in the other four groups - also to secure a desired assay sensitivity as well as be able to analyze all quantified using a mass spectrometer, a small amount of sample number and, by solving the analysis conditions other analysis conditions the two three required in a single HPLC injection detect the AIDS therapeutic agent that can reduce the time and cost waste by implementation of the conventional method, used in the clinical and screening ( there is an effect capable of screening). ; Therefore, to satisfy the present invention in the case of using the method for detecting the chemical properties the simultaneous analysis of a plurality of AIDS therapeutic agents belonging to the other group, with a small amount of a sample is also obtained for high assay sensitivity, accuracy, reproducibility, and rapidity and simplicity of analysis number, and accordingly it may know exactly the content of the AIDS therapeutic agents in plasma, it can be utilized to determine the appropriate indicators to determine the use of the therapeutic drug (dosage regimen) in AIDS treatment. ; AIDS treatment, high performance liquid chromatography, mass spectrometry, plasma.