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首页> 外文期刊>Journal of High Resolution Chromatography >Fast separation of nucleosides by capillary electrochromatography on non-endcapped phenyl-bonded silica phase using short-end injection method
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Fast separation of nucleosides by capillary electrochromatography on non-endcapped phenyl-bonded silica phase using short-end injection method

机译:短时进样法通过毛细管电泳在未封端的苯基键合硅胶相上快速分离核苷

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摘要

The influence of several experimental parameters (pH, ionic strength, organic modifier content of hydro-organic buffer) upon EOF, migration time, and retention factor has been studied in CEC with a phenyl-bonded silica column on a model mixture of five nucleosides. This paper illustrates the current interest in CEC as a method of resolving complex mixtures of neutral and ionic solutes and demonstrates the potential of the short-end injection method as a means of reducing analysis time. [References: 24]
机译:在带有五键核苷模型混合物的苯基键合硅胶色谱柱上,已经在CEC中研究了几个实验参数(pH,离子强度,有机缓冲液中有机改性剂的含量)对EOF,迁移时间和保留因子的影响。本文说明了目前在CEC中作为解决中性和离子性溶质的复杂混合物的方法的兴趣,并证明了短端进样方法作为减少分析时间的一种方法的潜力。 [参考:24]

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