Antimutagenic and Anticancer Effects of Leaf Mustard and Leaf Mustard Kimchi

摘要

In this study, we investigated antimutagenic and anticancer activities of leaf mustaid (LM, Brassica juncea) and leaf mustard kimchi (LMK) during their fermentation period. Methanol extracts were prepared from raw mustard, brined leaf mustard in 10% Gueun salt solution for 2 hrs, leaf mustard fermented at 15°C for 5 days after brined in 10% Guenun salt solution for 2 hrs (Fr-LM), fresh leaf mustard kimchi (Fresh-LMK) and optimally ripened leaf mustard kimchi fermented at 5°C for 30 days (OR-LMK). OR-LMK showed the strongest inhibitory activities against the mutagenicities induced by aflatoxin Bi in Salmonella Typhimurium TA100. LMs and LMKs inhibited the survival or growth of AGS human gastric adenocarcinoma cells and HT-29 human colon carcinoma cells in MTT assay and growth inhibition test Among the extracts, OR-LMK and FR-LM exhibited strong anti-proliferative effect against cancer cells, especially HT-29 cells. DAPI staining assay showed that OR-LMK induced apoptosis cell death of HT-29 cellsin a dose-dependent manner. These results suggest that leaf mustards and leaf mustaid kimchi have ehemopreventive activities.