Application of L-amino acid in determination of Huperzine A by high performance liquid chromatography

摘要

A selective HPLC method for determination of Huperzine A in Huperzia serrata Extract has been developed and validated. Huperzine A was dissolved in 0.01 mol/L HC1, chromatographed on an AgilentZorbax SB-C18 (150 mm x 4.6 mm i.d., mu m) column, with a mobile phaseconsisting of methanol-1 mM (L)-Lysine water solution (50:50, v/v), and detected at 310 nm. The UV peak areas were used for quantitation of Huperzine A content. The correlation coefficient (R-2) of the calibration was 0.9999 over the range of 1-25 mu g/ml and intra- and interday precision over this range were not more than 2%. The method was successfully applied to characterize and determine the Huperzine A in Huperzia serrata Extract. (c) 2006 Elsevier B.V. All rights reserved.