LC-ESI-MS/MS determination of in vivo metabolites of almotriptan in rat plasma, urine and feces: Application to pharmacokinetics

摘要

A highly sensitive and specific liquid chromatography-electrospray ionization tandem mass spectrometric (LC-ESI-MS/MS) method for investigating the in vivo metabolites of almotriptan in rat plasma, feces and urine was developed. Chromatographic separation was achieved on a Lichrospher RP-18 column (250mm×4.6mm, 5μm), using 20mM ammonium acetate (pH 3.5) and acetonitrile (60:40, v/v) as a mobile phase at 25°C. MS/MS detection was performed by positive ion electrospray ionization using target ions at m/z 336 [M+H] ~+, m/z 368 and m/z 282 [M+H] ~+ for almotriptan and its two metabolites, respectively. Two metabolites viz., γ-aminobutyric acid and sulfonamide were detected in plasma as well as feces after 24h of oral administration of almotriptan, while only γ-aminobutyric acid was found in urine. The method was sensitive with a lower limit of quantification of 1.43ng/mL and linear over the range of 1.43-5000ng/mL in plasma. The method was validated and successfully applied to a pharmacokinetic study of almotriptan in rat plasma using sumatriptan as an internal standard. The peak plasma concentration (C _(max)) after 0.3h of 5mg/kg oral dose of almotriptan was determined to be 69.85ng/mL.