Quantitative Comparison of Three Standardization Methods Using a One-Way ANOVA for Multiple Mean Comparisons

摘要

The use of an external standard calibration curve (1–3), standard addition (4, 5), and internal standard (6, 7) methods for quantitatively determining the concentration of analytes is important to the study of analytical chemistry,especially in the area of instrumental analysis. However, as important as these three methods are, few analytical or instrumental laboratory programs have the time for students to gain adequate laboratory experience with each of the three methods. The following laboratory experiment allows for students to gain experience with all three quantitative methods in a single laboratory period using the gas chromatograph (GC), and moves their exposure to these methods from traditionally scripted laboratory exercises to a more inquiry-based, comparative-learning experience (8).