Release Behavior and Biological Activity of Recombinant Human Bone Morphogenetic Protein-2 from Porous PLGA Scaffold

摘要

The feasibility of using the poly-(DL-lactic-co-glycolic acid) (PLGA) scaffold as a carrier for controlled recombinant human bone morpho-genetic protein-2 (rhBMP-2) delivery was assessed.PLGA rhBMP-2-loaded scaffolds were fabricated by emulsion polymerization and freeze-dried;a porous morphology was observed by scanning electron microscopy.The in vitro release of rhBMP-2 from the polymer was assessed using high performance liquid chromatography.An initial burst release of the incorporated rhBMP-2 was observed over the first 24 h followed by an 80.6% sustained release for one month.The rhBMP-2-loaded scaffolds were implanted bilaterally into rat ectopic muscle pouches.The rhBMP-2 implanted animals showed bone formation submuscle space and osteoblasts located in the new bone region while none was seen in the control groups.