Plant regeneration from anther derived callus cultures of ginger (Zingiber officinale Rosc.)

摘要

Ginger anthers collected at the uninucleate microspore stage were subjected to a cold treatment (0 deg) for 7 d and induced to develop profuse callus on MS medium supplemented with 2-3 mg l~(-1) 2, 4-D. Plantlets could be regenerated from these calli on MS medium supplemented with 5-10 mg l~(-1) BAP and 0.2 mg l~(-1) 2,4-D. The regenerated plantlets could be established in soil with 85% success, when they were planted in potting mixture of garden soil, sand and vermiculite in equal proportions and kept in a humid chamber initially for 22-30 d. This is the first report of successful regeneration of plants from ginger anther cultures and the protocol can be used for possible development of androgenic haploids and dihaploids in ginger.