Serum Immunoglobulin IgG Subclass Distribution of Antibody Responses to Yop Proteins and Lipopolysaccharide of Yersinia enterocolitica in Patients with Yersiniosis

摘要

To assess the humoral immunological responses at the IgG subclass level in yersiniosis specific antibody responses against lipopolysac-charide of Yersinia enterocolitica O3 (LPS) and Yersinia Yop proteins were analyzed by ELISA. Thirty five patients with arthritis and forty nine patients with uncomplicated yersiniosis were included in the study. Analysis of the IgG subclass responses to the LPS revealed that the subclass distribution for both groups of patients was IgG2>IgGl>IgG3. The concentration of IgG4 was below detection level. The predominant antibody responses to Yop proteins were IgGl>IgG3>IgG2>IgG4 but the frequency of detection of particular IgG subclass antibodies were dependent on the age of patients. Generally, the frequency of occurrence of IgG2 antibodies for Yop proteins of Yersinia together increased with age reaching its peak among individuals aged above 40 years. On the other hand, IgG1 for Yop proteins and IgG3 for Y. enterocolitica LPS were diagnosed more often in serum samplesobtained from children than from adults. We also found significantly higher frequency of IgG4 to Yop proteins of Y. enterocolitica in men than in women.