Regeneration of plantlets from the callus of stem explant of Hybanthus enneaspermus (L.) F. muell.

摘要

Callus-mediated shoot regeneration from stem explant of Hybanthus enneaspermus is described. Green compact nodular calli are obtained on MS medium containing 2.0 mg/l 2, 4-D and 0.5 mg/l BAP. Shoot buds were induced and elongated on MS basal medium with 5.0 mg/l BAP. The shoots were rooted in MS medium with 2.0 mg/l IBA, and then were successfully hardened and transferred to the field.