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Effects of calcium ions on β-glucan synthase activities in suspension-cultured cells of flax

机译:钙离子对亚麻悬浮培养细胞中β-葡聚糖合酶活性的影响

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摘要

Divalent ions, and especially calcium, highly stimulate glucan synthase activities. In thepresence of magnesium, glucan synthase GSI (EC 2.4.1.12) catalyses the formation of 1-3 and 1-4 glucosyl linkages in Golgi and low-density membranes. The addition of calcium ions reveals some glucan synthases of high affinity for UDP-glucose in plasmalemma and inhibits the activity detected in low density membranes. Moreover, the glucans synthesized in Golgi are more difficult to extract. On the other hand, glucan synthase GSII (EC 2.4.1.34) catalyses the formation of 1-3 glucosyl linkages in plasmalemma while it induces the formation of 1-4 glucosyl linkages in Golgi in presence of calcium.
机译:二价离子,尤其是钙,高度刺激葡聚糖合酶的活性。在镁的存在下,葡聚糖合酶GSI(EC 2.4.1.12)催化高尔基体和低密度膜中1-3和1-4葡糖基键的形成。钙离子的添加​​揭示了血浆中对UDP-葡萄糖具有高亲和力的某些葡聚糖合酶,并抑制了在低密度膜中检测到的活性。而且,在高尔基体中合成的葡聚糖更难以提取。另一方面,葡聚糖合酶GSII(EC 2.4.1.34)催化血浆中1-3个葡糖基键的形成,而在钙存在下诱导高尔基体中1-4个葡糖基键的形成。

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