Cytogenetic analysis of F1, F2 and BC1 plants from intergeneric sexual hybridization between Sinapis alba and Brassica oleracea by genomic in situ hybridization.

摘要

By intergeneric sexual hybridization between Sinapis alba and Brassica oleracea, F1, F2 and BC1 progeny plants were produced. S. alba plants (genome SS, 2n=24) were pollinated with B. oleracea (genome CC, 2n=18), and the fertile F1 plants were pollinated with B. oleracea to obtain BC1 plants. GISH analysis showed that 10 out of 12 F1 plants had 12 S. alba chromosomes (one full S chromosome set) and nine B. oleracea chromosomes (one C chromosome sets), representing the expected hybrids. However, two F1 plants had 12 S chromosomes and 18 C chromosomes (two C chromosome sets), indicating unexpected hybrids. A maximum of three trivalents between C and S chromosomes were identified at metaphase I of semi-fertile F1 pollen mother cells (PMCs), which indicates homology and chromosome pairing between these two genomes. The C genome had obviously been doubled in two F2 plants from selfed semi-fertile F1 plants. BC1 plants consisted of 18 C chromosomes and different numbers of one, five and six additional S chromosomes, respectively. Monosomic alien addition lines developed in the present study can be used for B. oleracea breeding and Sinapis alba gene mapping..