A linkage map of pak-choi (Brassica rapa ssp. chinensis) based on AFLP and SSR markers and identification of AFLP markers for resistance to TuMV.

摘要

A genetic linkage map for pak-choi, a very important economical and nutritional vegetable crop in Asia, has been constructed using amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers based on 180 F₂ individuals. It includes 133 AFLP and 74 SSR markers from 57 AFLP and 65 SSR primer combinations, respectively. Fifty SSR markers were previously used to construct a framework map and all AFLP markers and another 24 SSR markers were inserted. The linkage map consists of 10 linkage groups with a total distance of 1123 cM and an average interval of 5.43 cM. Turnip mosaic virus (TuMV) is one of the most important pathogens of pak-choi. TuRBCH01, a TuMV-C₅ resistance gene, was successfully mapped into R6 linkage group in the present genetic linkage map and was positioned between AFLP markers E36M62-3 and E44M48-1.Digital Object Identifier http://dx.doi.org/10.1111/j.1439-0523.2010.01811.x