INFLUENCE OF THE Mn(II) AND CH_4 ON THE EVOLUTION OF LIGHT INDUCED RADICALS IN CHLOROPLASTS

摘要

The photochemical activity of the leaves and chloroplasts of several species of plants developed in gaseous hydrocarbon environments (C_nH_(2n+2)) was investigated by the EPR method. The influence of different concentrations of paramagnetic ions [Mn(Ⅱ), Cu(Ⅱ) and VO(Ⅱ), respectively] on the plants grown in dietary media containing these ions was also followed by EPR spectroscopy. The contents of paramagnetic ions, lower than 0.1%, in the nutritive solutions, are the most suitable in the biosynthesis of chlorophyll-like pigments containing paramagnetic ions in their porphyrine ring. The changes in the form, structure and photosensibility of the EPR signals corresponding to the free radicals, as well as the properties of the pheophytins containing paramagnetic ions, such as: Mn(Ⅱ), Cu(Ⅱ) and VO(Ⅱ), were related to the photochemical synthesis in gaseous hydrocarbon environments.