Cationic microparticles: A potent delivery system for DNA vaccines

摘要

An approach involving the preparation of biodegradable micro- particles with a cationic surface was developed to improve the delivery of adsorbed DNA into antigen-presenting cells after i.m. injection. The microparticles released intact and functional DNA over 2 weeks in vitro. ln addition, the microparticles induced higher levels of marker gene expression in vivo. After i.m. immunization. the microparticles induced significantly enhanced serum antibody responses in comparison to naked DNA. Moreover. the level of antibodies induced by the microparticles was significantly en- hanced by the addition of a vaccine adjuvant, aluminum phos- phate. ln addition, in contrast to naked DNA, the cationic micro- particles induced potent cytotoxic T lymphocyte responses at a low dose.