In Vitro Therapeutic Potential of Leaf Extract of Eugenia uniflora linn on Acute - inflammation Rat Model

摘要

The leaves of Eugenia uniflora are used as anti-inflammatory and analgesic in the treatment of tissue injury in traditional medicine. The study evaluates the anti-inflammatory, analgesic, hematological and histological effects of ethanol extract of E. uniflora. The anti-inflammatory effect was accessed in test groups given different doses (50, 100 and 200 mg/kg, p. o) of the extract, a control group (0.1 mL/kg, normal saline) and standard drug group (Ibuprofen 100mg/kg, p. o) on formalin- induced paw edema. Body weight, paw weight, thickness and volume were evaluated. Analgesic property was accessed on separate group of animals by acetic acid-induced writhing and formalin-induced paw licking tests. Thereafter, hematological parameters were estimated and sections of the edematous skin were processed and stained with H and E. The Eugenia uniflora decreased paw volume and thickness after 3hr. There was dose dependent significant (P0.05) decrease in the number of abdominal writhing and insignificant reduction in the second or late phases of formalin-induced paw licking test group. Reduction in RBC, HB, PCV and increase in platelet, ESR, WBC were observed in the formalin-induced paw edema group. Histopathological assessment revealed aggregation of inflammatory cells in skin sections of the untreated group of the formalin-induced paw edema but a noticeable reversal in the extract and Ibuprofen treatment groups. These profiles indicate E. uniflora has potent anti-inflammatory and analgesic effects comparable to Ibuprofen a non steroidal anti-inflammatory drug (NSAIDs) and justifies it use in the treatment of inflammation and local tissue injury.