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Enly: Improving Draft Genomes through Reads Recycling

机译:Enly:通过阅读回收来改善基因组草图

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The reconstruction of the complete genome sequence of an organism is an important point for comparative, functional and evolutionary genomics. Nevertheless, overcoming the problems encountered while completing the sequence of an entire genome can still be demanding in terms of time and resources. We have developed Enly, a simple tool based on the iterative mapping of sequence reads at contig edges, capable to extend the genomic contigs deriving from high-throughput sequencing, especially those deriving by Newbler-like assemblies. Testing it on a set of de novo draft genomes led to the closure of up to 20% of the gaps originally present. Enly is cross-platform and most of the steps of its pipeline are parallelizable, making easy and fast to improve a draft genome resulting from a de novo assembly
机译:生物体完整基因组序列的重建是比较,功能和进化基因组学的重要意义。然而,在时间和资源上仍然需要克服在完成整个基因组的序列时遇到的问题。我们已经开发了Enly,这是一种基于重叠群序列读取的迭代映射的简单工具,能够扩展源自高通量测序的基因组重叠群,尤其是那些由Newbler类装配产生的重叠群。在一组从头起草的基因组上对其进行测试导致缩小了最初存在的缺口的20%。 Enly是跨平台的,并且其流水线的大多数步骤都是可并行的,从而使从头组装产生的基因组草图更容易,更快速。

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