Improved genome editing with programmable nuclease and improved genome editing without programmable nuclease

摘要

The present invention includes compositions and methods for genome editing in isolated cells or organisms. This editing oligonucleotide comprises an oligonucleotide chain that can contain a linker that positions the editing moiety at an appropriate position to modify the target nucleic acid, a crispr RNA domain that causes deamination of the target nucleobase, and an inactivated Cas9 domain Contains The editing oligonucleotides may also contain at least one nucleotide sequence variation from the target sequence in the genome. A particular embodiment of the method involves modifying the genomic sequence in the cell utilizing editing oligonucleotides without exogenous proteins that aid the editing process. The editing oligonucleotide may comprise a backbone modification that increases the nuclease stability of the oligonucleotide relative to the unmodified oligonucleotide.