gene expression construct, gene expression system, variance of a gene expression system, process for increasing expression or enhancing the translational activity of a sequence derived from a rna-2 genome segment of a bipartite virus, methods to express a protein of interest in a host organism, to improve translation of a heterologous protein of interest from a gene or open reading frame, to express a protein gene expression construct, gene expression system, variation of a system Gene Expression, A Process for Increasing Expression or Improving the Translational Activity of a Sequence Derived from a Rna-2 Genome Segment of a Bipartite Virus, Methods for Expressing a Protein of Interest in a Host Organism, to Improve Translation of a protein of heterologous interest of an open reading frame or gene to express a protein in a plant and plant to generate a protein of interest, and host organism

摘要

The invention is based on an expression enhancer sequence derived from the RNA-2 genome segment of a bipartite RNA virus, in which a target initiation site in the RNA-2 genome segment has been mutated. Deletion of appropriate start codons upstream of the main RNA2 translation initiation can greatly increase in foreign protein accumulation without the need for viral replication. Also provided are methods, vectors and systems, including the ‘hyper-translatable’ Cowpea Mosaic Virus (“CPMV-HT”) based protein expression system.