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Quantification of Caffeoylquinic Acids in Coffee Brews by HPLC-DAD

机译:HPLC-DAD定量分析咖啡冲泡中的咖啡酰奎尼酸

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The influence of different brewing conditions on the concentration of the main caffeoylquinic acids (3-caffeoylquinic acid (3-CQA), 4-caffeoylquinic acid (4-CQA), and 5-caffeoylquinic acid (5-CQA)) was investigated. For this purpose, twenty-four coffee brews were extracted and analyzed using HPLC-DAD at 325 nm. Our findings demonstrate the great impact of brewing techniques on the caffeoylquinic acids (CQAs) content. The major isomer was 3-CQA, accounting for about 50% of the total CQAs, followed by 5-CQA and 4-CQA, accounting for about 24–36% for each one. The total content of CQAs was in the range of 45.79 to 1662.01 mg/L, found in iced cappuccino and pod espresso, respectively. In conclusion, this study demonstrates that coffee brews, in particular those prepared using pressurized methods, can be considered as the potential sources of antioxidants such as CQAs.
机译:研究了不同酿造条件对主要咖啡酰奎尼酸(3-咖啡酰奎尼酸(3-CQA),4-咖啡酰奎尼酸(4-CQA)和5-咖啡酰奎尼酸(5-CQA))浓度的影响。为此,提取了24种咖啡冲剂,并使用HPLC-DAD在325nm处进行了分析。我们的发现证明了酿造技术对咖啡酰奎尼酸(CQA)含量的巨大影响。主要异构体是3-CQA,约占总CQA的50%,其次是5-CQA和4-CQA,每种约占24-36%。 CQA的总含量在45.79至1662.01 mg / L的范围内,分别存在于冰镇的卡布奇诺咖啡和豆荚浓缩咖啡中。总之,这项研究表明,咖啡冲泡剂,特别是使用加压方法制备的冲泡剂,可以被视为潜在的抗氧化剂来源,例如CQA。

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