Lactobacillus rhamnosus Ability of Aflatoxin Detoxification

摘要

The present study was conducted to isolate and identify Lactobacillus rhamnosus from locally fermented dairy products collected from different markets in Irbid city, Jordan. Thereafter, the ability of Lb. rhamnosus to detoxify aflatoxins (AFs) was investigated in vitro after incubation on 37°C in MRS medium and in artificial intestine fluid (AIF). Three Lb. rhamnosus out of nine different species of Lactic Acid Bacteria (LAB) isolated from 15 fermented dairy products samples were identified. The isolates were characterized based on their morphological, microscopic, cultural and biochemical properties. The selection of isolates as probiotics depended on their abilities to grow in pH levels between 2 to 6 and their tolerance to grow at 1.0 % bile salts concentrations, Furthermore, Lb. rhamnosus was able to adhere to mucus onto the intestine surface at 54.7%. The ability of Lb. rhamnosus of AFs detoxification has significantly (p0.05) increased with the increase in incubation periods, and the detoxification percentage after 72h incubation in each MRS medium and AIF, was 76% and 81.6%, respectively.