Molecular Characterization of Multidrug-Resistant Bacteria Isolated from a Boyacá Hospital, Colombia

摘要

There is a growing number of beta-lactamase extended spectrum bacteria, which inactivate a wide variety of beta-lactam drugs (ESBLs), including third-generation cephalosporins, penicillins, and monobactams, which are commonly identified in nosocomial infections. Genetic variability and molecular approaches have been widely used for bacterial phylogeny and have been applied in bacterial identification. The bacterial isolates were obtained from June 2014 to December 2014 in a second-level hospital in the state of Boyacá-Colombia. Phenotypic identification was performed using the BioMerieux VITEK-2 system? following the standards of the Institute of Clinical and Laboratory Standards. ESBLs genes, including TEM, SHV, CTXM and AmpC, were analyzed, and 11 strains with drug resistance were analyzed. From the isolates, 100% expressed the blaCTX gene, while 18.2% expressed blaSHV and 27.3% expressed blaTEM. The researchers observed two different amplifications of AmpC genes, one that was 170 bp and the other that was 500 bp. The first had a frequency of 100% in the samples and the second had a frequency of 63.6%. Bioinformatic analysis was performed for each sequence. ESBLs-producing strains are considered a major problem due to multi-drug resistance, their implications in nosocomial outbreaks, and their tendency to spread rapidly throughout the world. Therefore, they are considered important clinical markers; consequently, the knowledge of incidence and origin play an important role in the selection of appropriate treatment.