Cloning and expression analysis of fatty acid desaturase gene NtSAD1 in Nicotiana tabacum

摘要

Tobacco K326 (Nicotiana tabacum L.) was selected as experimental material, and a △9 Stearoyl-ACP desaturase (NtSAD) gene was cloned from the leaf by RT-PCR, which was named as NtSAD1. Homology analysis showed that NtSAD1 gene shared high homology with other plant SAD genes and the higher homology among them include tomato 79.71%, Jatropha curcas 79.32%, potato 79.04% and castor bean 79.00%, respectively. It was found that the expressions of NtSAD1 in leaves, roots and stems were the highest at blossom stage, separately. And the expression of NtSAD1 in tobacco leaves was the highest in all organs. These results suggest that it is related to oil of tobacco leaves.