Effects of Temperature and Acetonitrile on Microwave-Assisted Weak Acid Protein Hydrolysis

摘要

The effects of temperature and acetonitrile (ACN) concentration on microwave-assisted weak-acid hydrolysis of proteins were investigated. Myoglobin was hydrolyzed for 1 h using 2% formic acid and a microwave with different concentrations of ACN (0, 5, and 10%) at various temperatures (50, 60, 70, 80, 90, and ). The numbers of peptides identified with each concentration of ACN were the same for each temperature. The greatest number of peptides (18 total) was obtained with hydrolysis at , and 6 of these were a result of additional removal of aspartic acid at the C-terminus. Hydrolysis at resulted in 13 peptides, of which only 1 was generated by the additional removal of aspartic acid, and 12 were observed with hydrolysis at . Our results demonstrate that microwave-assisted weak-acid hydrolysis of proteins can be performed successfully at , which could be beneficial for limiting side reactions and generating larger peptide sequences.