Protection of DNA and Erythrocytes from Free Radical Induced Oxidative Damage by Methanolic Extract of Amla (Phyllanthus emblica)

摘要

Amla (Phyllanthus emblica) is well known for its rich Vitamin C and polyphenolic contents which contribute to its antioxidant activity. The present study was focused on the total phenolic, proanthocyanidin, flavonoid contents and also protective effect of phenolic compounds present in methanolic extract of amla against free radical induced oxidative damage on DNA and erythrocytes. The extract was also tested for its inhibitory action on α- glucosidase and its reducing power. The total phenolic content was evaluated to 108.4 mg gallic acid equivalent (GAE)/g. The amount of flavonoids and proanthocyanidin content were recorded as 40.1 mg quercetin equivalents (QE)/g and 60.4 mg catechin equivalents (CE)/g respectively. The extract prevented the DNA from damage, after treatment of λ DNA with H2O2 and methanolic extract of amla. The oxidative hemolysis induced by hydrogen peroxide in rat erythrocytes was inhibited by amla extract in a dose dependent manner. The methanolic extract of amla exhibited good antioxidant properties which was comparable with standard antioxidant used.