The purification and identification of heme oxygenase isoforms form spleen tissue of rat and the expression of heme oxygenase-1 cDNA in COS-1 cells

摘要

Conclusion The activity of expressed HO-1 in COS-1 cells is higher than that of purified enzyme from rat spleen tissue. It is suggested that this clone having an insert of 1030 base-pairs encodes HO-1 and that we can prepare HO-1 mutant by site-directed mutagenesis Of HO-1 cDNA to prevent and treat hyperbilirubinemia. Objective To demonstrate that heme oxygenase (HO) isoforms exist in rat spleen treated with hematin and phenylhydrazine and to confirm that the isolated cDNA actually encodes HO-1 by expressing cDNA in monkey kidney cells (COS-1 cells) in order to prepare HO-1 mutant for inhibiting the natural enzyme.