Sublethal Irradiation Promotes Migration and Invasiveness of Prostate Cancer PC-3 Cells:lmplications for Radiotherapy of Human Prostate Cancer

摘要

OBJECTIVE To study the changes in the matrix metalloproteinases-2 and 9 (MMP2, MMP9) induced by ~(60)Co γ-ray external irradiation of human prostate cancer PC-3 cells. METHODS Human prostate cancer PC-3 cells were irradiated with different doses of ~(60)Co γ-rays. Cell migration and invasiveness were evaluated and the expression of MMP2, and MMP9 was investigated by RT-PCR, Western blotting and flow cytometry(FCM). RESULTS Irradiation enchances invasive protential at the doses of 1,3 and 5 Gy, whereas it significantly inhibits cell migration. CONCLUSION The different doses of ~(60)Co γ-ray external irradiation for prostate cancer may have different effects through the changes of MMP2, and MMP9 expression.