IRRA-Spektroskopie: Wechselwirkung von Proteinen mit Lipidmonoschichten Lipid-Protein-Wechselwirkung

摘要

Lipid monolayers at the water/air interface mimic half of a bilayer lipid membrane. Infrared reflection-absorption spectroscopy (IRRAS) is ideally suited to study the interaction of proteins with lipid monolayers and to determine changes in secondary structure of the proteins or peptides occurring upon binding to the lipid monolayer. From IRRA-spectra taken with linearly polarized light at different angles of incidence the determination of the orientation of secondary structure elements is possible.