Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter

摘要

The glucose-import system of Escherichia coli consists of a hydrophilic EIIAGlc subunit and a transmembrane EIICBGlc subunit. EIICBGlc (UniProt P69786) contains two domains: the transmembrane EIICGlc domain (40.6 kDa) and the cytoplasmic EIIBGlc domain (8.0 kDa), which are fused by a linker that is strongly conserved among its orthologues. The EIICBGlc subunit can be split within this motif by trypsin. Here, the crystallization of the tryptic EIICGlc domain is described. A complete data set was collected to 4.5 Å resolution at 100 K.