A modified method for enzymatic isolation of and subsequent wax extraction from Arabidopsis thaliana leaf cuticle

摘要

Design of an optimised method for enzymatic isolation of Arabidopsis leaf cuticle. Fresh leaf discs floating on solution surface (a) and digested leaves with isolated cuticles after approximately 1 week of isolation (b) are shown. Silicone annuli were used to keep cuticle discs floating on the water surface, whereas digested tissue sank down