Amyloid precursor protein glycosylation is altered in the brain of patients with Alzheimer’s disease

摘要

Increased APP mRNA in the frontal cortex of AD patients. Relative mRNA expression of transcripts for a total APP, and the b APP-KPI and APP-695 splice variants, analyzed by qRT-PCR in frontal cortex tissue from NDC (n = 7) and AD subjects (Braak stages V–VI, – = 7). To analyze APP transcripts, specific Power SYBR® Green PCR Master Mix primers were employed and the specificity of the PCR products was confirmed by analyzing the dissociation curves. Transcript levels were calculated by the comparative 2−ΔCt method with respect to 18S rRNA from the same cDNA and expressed as the mean ± SEM: p < 0.001 relative to the NDCs as indicated