Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis

摘要

Number of immune cells and level of OVA-specific IgE in BALF. Experimental scheme for OVA-induced asthma model and BAW treatment. After the collection of BALF from the lungs, total cells were separated by centrifugation, and stained with May-Giemsa solution. Total cells, eosinophils and macrophages were measured within a 1 mm area under a light microscope at 400× magnification. The concentration of OVA-specific IgE was quantified in BALF by an enzyme-linked immunosorbent assay (ELISA). The minimum detectable concentration of this kit is 20.7 pg/mL. The data shown represent the means ± SD of duplicates. *,  P