苹果多酚对卡介苗联合脂多糖所致小鼠免疫性肝损伤的保护作用

摘要

Objective:To observe hepatoprotective role of apple polyphenols (AP,Appjfnol )on immunological liver injury in mice. Methods:An immunological liver injury model in mice was established by intravenous injection of Bacillus Calmette-Guerin (BCG)and lipopolysaccharide (LPS).The liver index and spleen index,the activities of serum aspartate aminotransferase (AST)and alanine ami-notransferase (ALT),the levels of serum total protein (TP),albumin (Alb)and albumin/globulin (A /G)were detected.Histopatho-logic change was also investigated by HE staining.Results:AP inhibited the increases of liver index and spleen index,prevented the in-crease of serum AST activity and the decrease of serum TP and Alb levels.Histopathological abnormalities were also improved after AP administration.Conclusion:AP played a significant hepatoprotective role against BCG and LPS induced immunological liver injury in mice.%目的：观察苹果多酚（Apple Polyphenols，AP）对小鼠免疫性肝损伤的保护作用。方法：采用卡介苗联合脂多糖制备小鼠免疫性肝损伤模型。研究 AP 对小鼠肝指数和脾指数，血清中谷草转氨酶（Aspartate Transaminase，AST）和谷丙转氨酶（Alanine Transaminase，ALT）活性，血清总蛋白（Total Protein，TP），白蛋白（Albumin，Alb）和白蛋白/球蛋白（A /G）含量的改善作用，同时采用 HE 染色方法观察 AP 对免疫性肝损伤小鼠肝脏病理改变的保护作用。结果：AP 能明显抑制免疫性肝损伤模型小鼠异常升高的肝指数和脾指数，降低血清中 AST 活性，不同程度地升高血清中 TP 和 Alb 的含量，同时对肝组织病理异常变化也有显著的改善作用。结论：AP 对卡介苗联合脂多糖所致的小鼠免疫性肝损伤具有保护作用。