Objective:To study the best preparation process of chitin extraction from protaetia brevitarsis , Lewis.Methods:The a-dult larvae of the white star flower are used as raw materials , and the adult powder and protein are removed by strong acids and al-kalis.The chitin is obtained after decolorization.The residual inorganic salts and protein in the extract are determined by dry ash method and ultraviolet absorption method.Results:1.5 g/L citric acid solution was used to soak adult protaetia brevitarsis , Lewis powder at a ratio of 1∶10 at room temperature for 26 h, and the effect of removing inorganic salts was better ; 10%NaOH solution was used to the ratio of material to liquid was 1∶10, and the extract was processed at 80 ℃ water bath for 4.5 h.The effect of deproteinization was better.The white powdery chitin was obtained by using 10%H2O2solution at a ratio of 1∶12 in a water bath at 60 ℃for 3 h.The yield rate was 28.3%.Conclusion:The extraction process is simple and feasible , and the obtained chitin has high yield, good traits and low residual protein and ash content.%目的:探讨从白星花金龟中提取甲壳素的最佳制备工艺.方法:选取白星花金龟的成虫为原料,采用强酸强碱对成虫粉进行脱除无机盐和蛋白质处理,再脱色后得到甲壳素,用干法灰分法和紫外吸收法分别测定提取物中残余的无机盐和蛋白质.结果:用1.5 g/L柠檬酸溶液在室温下按料液比1∶10浸泡白星花金龟成虫粉26 h,脱无机盐效果较好;用10%氢氧化钠溶液按料液比为1∶10在80 ℃水浴条件下处理提取物4.5 h,脱蛋白质的效果较好;用10%H2O2溶液按料液比为1∶12在60℃水浴浸泡3 h脱色,可制得白色粉状甲壳素,得率为28.3%.结论:该提取工艺简便可行,获得的甲壳素得率高,性状良好,残余蛋白质和灰分含量较低.
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